Anand Ramasubramanian, Ph.D.

AnandRamasubramanian

Associate Professor

Email

Preferred: anand.ramasubramanian@sjsu.edu

Telephone

Preferred: 408-924-3922

Office

E385H
San José State University
One Washington Square
San José, CA 95192-0085

Education

  • B.S. (Chemical Engineering, Annamalai University, India)
  • M.S. (Chemical Engineering, Indian Institute of Science, India)
  • Ph.D. (Bioengineering, Rice University, Houston, TX)

Bio

Academic Appointments

  • Associate Professor (tenured) of Biomedical Engineering (University of Texas at San Antonio)
  • Post-doctoral Scholar (Chemical Engineering, University of California, Berkeley)

Honors and Awards

  • Whitaker Foundation Fellowship, Rice University
  • Government of India Fellowship
  • Gold Medal for best academic performance, Annamalai University, India

Teaching Interests

  • Process Dynamics and Control (Spring 2017)
  • Chemical Reactor Design (Fall 2016)
  • Applied Mathematics
  • Biotransport Phenomena

Research and Scholarly Interests

• Vascular mechanobiology
• High-throughput assay development

Current Research

  • Platelet storage for transfusion

Platelets are transfused to prevent bleeding and induce hemostasis, and can thus be critical in saving lives following trauma. Currently, platelets isolated from volunteers are stored at room temperature (RT) with gentle agitation for up to 5 days, before transfusion. This short shelf-life severely compromises platelet inventories and creates chronic shortages with the major issue being bacterial contamination. To address this issue, we are pursuing refrigerated platelets as a viable product for transfusion. We have shown that refrigerated platelets are superior to platelets stored at RT under standard blood-banking conditions by several metrics: metabolic and hemostatic functions, response to physiologic inhibitors, and clot mechanical properties. Our work, in collaboration with the U.S. Army, has recently prompted the FDA to clarify that cold-stored platelets may be used as a therapeutic product for active hemorrhage.

  • High-throughput antimicrobial drug discovery and diagnostics

Infectious diseases are still the leading cause of death in the world as new organisms and drug resistance strains emerge. There is an urgent need for early detection and targeted treatment of the pathogens. To fill this technology gap, we have developed a miniaturized microbial culture platform that has cut down the time, cost, and reagent use. Our platform consists of several thousand spots of 3-dimensional, 30 nL cultures of single or multiple bacterial (Staphylococcus aureus, Pseudomonas aeruginosa) and fungal (Candida albicans) species grown on glass or paper substrates. We have shown that despite more than 3000-fold reduction in volume, our nano-scale cultures on the chip are comparable to current industry standards. We have used this screen to identify novel drugs and their combinations with effective antimicrobial activity. We have also modified this platform for a rapid and inexpensive point-of-care for testing drug efficacy.

  • Role of fluid shear stresses and transport on inflammatory response

The focus of medicine has been on chemical factors as the chief determinant of disease development and treatment. Recently, we have come to recognize that physical factors such as mechanical forces can be just as important. We have investigated the role of fluid shear stresses and transport due to blood flow on infection and inflammation. Using in vitro microscale models of the blood vessels, we have shown that physiological levels of fluid shear stress can significantly upregulate pro-inflammatory responses from monocytes infected with Chlamydia pneumoniae, a pathogen implicated in atherosclerosis. In another study, we have shown that blood flow can monocytes assist in the adhesion of the otherwise non-adherent metastatic breast tumor cells to the microvascular endothelium under flow thus increasing the chances of hematogenous metastasis.

Selected Publications

  1. Evani, S.J., S.F. Dallo, A.K. Ramasubramanian (2016), “Biophysical and biochemical outcomes of Chlamydia pneumoniae infection promotes pro-atherogenic matrix microenvironment”, Frontiers in Microbiology, August, 7:1287. PMID: 27582738.
  2. Srinivasan, A., J.L. Lopez-Ribot, A.K. Ramasubramanian (2015), “Microscale microbial cultures”, Future Microbiology, 10:143-6. Editorial.
  3. Reddoch, K.M., H.F. Pidcoke, R.K. Montgomery, C.G. Fedyk, A.K. Ramasubramanian*, A.P. Cap* (2014), “Hemostatic capacity of apheresis platelets is better preserved by storage at 4 °C than 22 °C”, Shock, 41: 54-61 (*Co-corresponding authors). PMID: 24169210.
  4. Evani, S.J., R.G. Prabhu, V. Gnanaruban, E. Finol, A.K. Ramasubramanian (2013), “Monocytes mediate metastatic breast tumor cell adhesion to endothelium under flow”, FASEB J., 27(8):3017-29. PMID: 23616566.
  5. Srinivasan, A., J.L. Lopez-Ribot, A.K. Ramasubramanian (2012), “Candida albicans Biofilm Chip (CaBChip) for high-throughput antifungal drug screening”, Journal of Visual Experimentation, 65: e3845. PMID: 22847237.
  6. M.-Y. Lee*, Kumar, R. A.*, M. Hogg, S. Sukumaran, J.S. Dordick, D.S. Clark (2008), “Three-dimensional cellular microarray for high-throughput toxicology assays”, Proc. Natl. Acad. Sci. (USA), 105, 59-63 (* equal contribution). PMID: 18160535

Recent Funding

  1. Systems biology based tools for modeling platelet storage lesion for optimal blood transfusions (PI), DOD
    SBIR Phase II Subcontract, 10/01/2016-09/30/2018
  2. The role of factor XIII on platelet contractility (PI)
    CCCRP, MRMC, US Army, 10/1/2016-09/30/2019
  3. Interaction between fluid shear and Chlamydia pneumoniae infection in atherosclerosis (PI)
    SC1, NIH 

Professional Service

  1. Editorial Board, Frontiers in Microbiology
  2. Reviewer
    Acta Biomaterialia, Annals of Biomedical Engineering, Biotechnology & Bioengineering, British J. Cancer, Cell Motility and Cytoskeleton, Experimental Cell Research, Journal of Biomedical Materials Research, Cellular and Molecular Bioengineering, Lab-on-a-Chip, Frontiers in Microbiology, Microbial Pathogenesis, PLoS One, Tissue Engineering, Future Medicinal Chemistry
  3. Ad-hoc grants reviewer
     •  American Heart Association
     •  National Institutes of Health
  4. Director of Undergraduate Program in Biomedical Engineering, 2014-2016, UTSA
  5. Chair of Task force for new BS program in Chemical Engineering, 2014-2016, UTSA

Students

B.S. students
  1. Amir Dastgheib
  2. Andrew Tran
  3. Kevin Hernandez
  4. Stephen Schobey
M.S. students
  1. Abed Abugherir
  2. Salvador Mireles