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Steven B. Lee

Lee, Steven B.

Professor,  Justice Studies
Director Forensic Science, Justice Studies

E-mail
sblee999@gmail.com
steven.lee@sjsu.edu
Additional Contact Information

Phone Number(s)
(408) 924-2948

Office Hours
Spring 2014 Office Hours In person:Weds 1300-1500 by email appointment and Fridays on line 1300-1500
Fall 2013 Office Hours In person: Thurs 1300-1500 by email appointment and On line Fridays 1300-1500

Courses

Education

  • Doctor of Philosophy. Univ Of Cal-Berkeley, 1990
  • Master of Science, Molecular Biology
    New York Univ, United States, 1984
  • Bachelor of Science, Biological Sciences
    Suny At Binghamton, United States, 1981

Bio

Research Description

Forensic Molecular Biology is the molecular analysis of biological evidence to provide objective information on legal matters or those that pertain to criminal and civil law. The different types of biological evidence may include human bodily fluids or tissues and/or non-human materials such as botanical, fungal, entomological, microbiological or zoological specimens. Comparison of DNA profiles generated from the biological evidence at the crime scene to known samples can serve to link suspects with crime scenes or victims or can exonerate the suspect. Forensic molecular biologists use a variety of tools and techniques to examine and characterize biological evidence. These include microscopy, presumptive chemical testing, immunological analyses, genotyping of autosomal and Y chromosome micro satellite multiplexes (or short tandem repeats -STR), sequencing of mitochondrial DNA hypervariable regions I and II and more recently the use of single nucleotide polymorphism for ethnic profiling and messenger RNA for tissue typing. Results of the STR tests can be compared to unknowns or to a national DNA database, CODIS (Combined DNA Index System) that currently contains over 7.7 million profiles. These comparisons can lead to “cold hits” that provide the ability to solve crimes in cases in which there are no known suspects.

Research in my laboratory has fallen into three general categories:

1. DNA sample collection, archiving and storage strategies for genetic repositories and databases.

2. Y chromosome marker evaluation and testing

3. Forensic DNA PCR optimization and DNA Repair

Relevant publications:

Lee SB and KA Roberts. 2008. Saving your Samples. Emerging Technologies in Forensic Science. 01/08 p 8-10.

Lee, SB. 2006. Forensic DNA testing. In McGraw-Hill 2006 Yearbook of Science and Technology . ISBN 0071462058. McGraw-Hill.

Kimberly Clabaugh, Brie Silva, Kingsley Odigie, Ron Fourney, Jesse Stevens, George Carmody, Mike Coble, Odile Loreille, Melissa Scheible, Margaret Kline, Thomas Parsons, Arijana Pozder, Arthur Eisenberg, Bruce Budowle and Steven B. Lee. 2007. Storage of DNA samples at ambient temperature using DNA-SampleMatrix. Poster presented at the 18th Annual International Symposium on Human Identification. held October, 2007. Hollywood, CA.

http://www.promega.com/geneticidproc/ussymp18proc/abstracts/Abstract48Clabaugh.pdf Also presented as an oral presentation at the Fall 2007 California Association of Criminalists Meeting held in Berkeley, CA October 2007.

Arturo Aguilar, Kimberly Clabaugh, Anthony Carter, Sudhir Sinha and Steven B. Lee. 2007. Development of a male DNA Screening Assay using Y Alu Derived sequences for detection on the FMBIO III plus. Poster presented at the 18th Annual International Symposium on Human Identification. held October, 2007. Hollywood, CA.

http://www.promega.com/geneticidproc/ussymp18proc/abstracts/Abstract84Aguilar.pdf Poster presentation at the 2007 Society for the Advancement of Chicano and Native American Scientists meeting held Kansas City, MO, October 11-14, 2007. Also presented as an oral presentation at the Fall 2007 California Association of Criminalists Meeting held in Berkeley, CA October 2007.

Kingsley Odigie, Lynne Burley, Ian Fitch and Steven Lee. 2007. Repair of damaged DNA using commercially available enzymes. Poster presented at the 18th Annual International Symposium on Human Identification. held October, 2007. Hollywood, CA.

http://www.promega.com/geneticidproc/ussymp18proc/abstracts/Abstract38Odigie.pdf Poster presentation at the 2007 Society for the Advancement of Chicano and Native American Scientists meeting held Kansas City, MO, October 11-14, 2007. Also presented as an oral presentation at the Fall 2007 California Association of Criminalists Meeting held in Berkeley, CA October 2007.

R. Muller, J. Muller-Cohn and SB Lee. 2007 Optimizing DNA Stabilization and Storage of Forensic DNA Samples using polymers. Poster presented at the 18th Annual International Symposium on Human Identification. Held October, 2007. Hollywood, CA. http://www.promega.com/geneticidproc/ussymp18proc/abstracts/Abstract16Lee.pdf

Hirofumi Fukushima, Steven B. Lee, Yoshiya Fukuma, Masamitsu Nakamura, Hiroaki Yoshida and Takahiko Kasuga. 2007. Overview of a lab support system for managing data from DNA. Poster presented at the 18th Annual International Symposium on Human Identification. Held October, 2007. Hollywood, CA http://www.promega.com/geneticidproc/ussymp18proc/abstracts/Abstract10Fukuma.pdf

Silva, B, C. Cook, S. Murillo and S.B. Lee. 2007. Evaluation of Zeolite as a substrate for Collection and Storage of DNA. Abstract accepted and poster at the 2007 American Academy of Forensic Sciences meeting held February 2007 San Antonio, TX, and a poster at the 2006: 17th Annual International Symposium on Human Identification. Held on October 9-12, 2006. Nashville TN

Villa, E, K. Ballinger, A. Carter and S.B. Lee. 2006. Development of a Y chromosome specific screening method for sexual assault evidence. Abstract and poster presentation at the Society for Advancement of Chicanos and Native Americans in Science National Conference. Held on October 26-29, 2006. Tampa Fl.and presented as a poster at 17th Annual International Symposium on Human Identification. Held on October 9-12, 2006. Nashville TN

Matsumoto, T, R. Nakashige1, and S. B. Lee. 2006. Expert System for Evaluating Automated Allele Called Data. Abstract and poster at the 17th Annual International Symposium on Human Identification. Held on October 9-12, 2006. Nashville TN.

Murrillo, S and S. Lee 2006. Recovery of DNA from unique substrates. Poster presented at the 2006 American Academy of Forensic Sciences meeting. Seattle, WA, Feb. 20-25, 2006. Forensic Science Foundation Ribbon Award.

Lee, S and S. Murillo. 2006. Evaluation of Zeolite and Chitosan as Substrates for Collection and Storage of DNA. Spring CAC Conference oral presentation. Abstract. CACNEWS. Third Quarter 2006 p. 28. http://www.cacnews.org/pdfs/3rdq06.pdf– Awarded the 2006 A.Biasotti Most Outstanding Research Presentation.

Lee, SB. 2006. Forensic DNA Research and Teaching at San Jose State University: Establishing Partnerships in Academia, Biotechnology, and Government. Oral presentation at the 2006 American Academy of Forensic Sciences meeting. Seattle, WA, Feb. 24, 2006.

Lee, SB. 2005. Forensic Biology. In McGraw-Hill 2006 Yearbook of Science and Technology . ISBN 0071462058. McGraw-Hill. 448pp, DOI: 10.1036/0071462058

Lee, SB. 2005. Teaching Strategies in Undergraduate Forensic Science and DNA: New Approaches to Content, Assessment and Practice. Canadian Journal of Police and Security Services. 3(2):27-36.

Lee, SB, A.T. Minn, T. Nagai, T Shimizu, F. Ogura and M. Kuji. 2005. Integrated acquisition and analysis of multiplexed immuno- and SNP assays using the Masterplex software suite. Poster presented at the Luminex X Map symposium. Held April 25-27, 2005. Austin, TX. http://www.luminexcorp.com/planetxmap/Minn.pdf

Larsen, K and S. Lee. 2005. Optimization Strategies for DNA storage. Abstract accepted for the 16th Annual International Symposium on Human Identification. 26-29 September 2005. Grapevine, TX

Smith, K and S Lee. 2004. Comparison of DNA storage methods. Poster presentation at the 15th International Symposium on Human Identification. October 4-7, 2004. Scottsdale, AZ. http://www.promega.com/geneticidproc/ussymp15proc/posterpresentations/56Smith.pdf

Lee, SB. 2002. Biodefense: Science Steps Up to Protect the Public from Bioterror. Drug Discovery and Development. Dec. 2002. p 12. Invited Editorial.

Budowle, B, *W. Hudlow, S Lee, and L Klevan. 2001. Using a CCD Camera Imaging System as a recording device to Quantify Human DNA by Slot Blot Hybridization. Biotechniques. 30:680-685.

Worley, J. W., S.B. Lee, *M. Ma, A. Eisenberg, and E.S. Mansfield. 1997. Fluorescent Imaging in Human Identity Testing. Biotechniques. 23:148-153

Butler, JM, Jia Li, Joseph Monforte, Christopher Becker, and Steven Lee. 1997. Rapid and Automated Analysis of Short Tandem Repeat Loci Using Time-of-Flight Mass Spectrometry. . Proceedings of the Seventh International Symposium on Human Identification. ISBN1-882274058-X http://www.promega.com/geneticidproc/ussymp8proc/28.html

Post, S, S. Lee, T. Moretti, J. Robertson, and B. Budowle. 1997. Comparison of Fluorescence Detection of the Geneprint Powerplex STR System by Optical Scanners and DNA Sequencers. Proceedings of the Seventh International Symposium on Human Identification. ISBN1-882274058-X. pp. 107-115. http://www.promega.com/geneticidproc/ussymp7proc/0716.html

Lee, SB, M. Buoncristiani, J. W. Schumm, and D. Wingeleth. 1996. Comparison of Short Tandem Repeat (STR) detection using silver, fluorescence and matrix assisted laser desorption/ionization time-of-flight mass spectrophotometry (MALDI-TOF-MS). Proceedings of the Sixth International Symposium on Human Identification. ISBN 1-882274-55-5 p104-111. http://www.promega.com/geneticidproc/ussymp6proc/lee.htm

Micka, K.A., C.J. Sprecher, A.M. Lins, C.T. Comey, B.W. Koons, C Crouse, D. Endean, K. Zold, S.B. Lee, N. Duda, *M Ma and J.W. Schumm. 1996. Validation of Multiplex Polymorphic STR Amplification Sets Developed for Personal Identification Applications. Journal of Forensic Sciences. 41: 582-590.