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JS 111 Fluorescent Applications in Molecular Bio and Forensic Sci

Time: Monday, Wednesday 1:30pm-245pm
Location: TBD

Description

This course covers scientific concepts, methods, practices, instrumentation, interpretation, statistics and court issues of fluorescence applications in molecular biology and forensic science. Atomic and molecular basis of fluorescence, emission, photophysical processes, energy transfer, QA, validation, accreditation, and admissibility will be covered in lectures, hands-on activities/laboratories, videos and guest lectures.

110810 Dr. Pourmand Guest lecture

Dr. Nader Pourmand advanced methods in forensic dna

111010 SNP Powerpoint

SNP Powerpoint PDF

FRET review article

Fret review article due 101610

Review of DNA PCR, FRET

100410 powerpoint on intro to dna

102010 Multiplex STR PCR powerpoint

102010 Multiplex PCR presentation

Intro to DNA, PCR and qPCR powerpoint

Intro to DNA PCR and qPCR

Laser lecture 3 by Dr. Stone

Laser Lecture 10/11/10

FRET applications powerpoint grad

102510 FRET applications powerpoint

New assignment 091310

Due 091510 1.Group assignments- Blood (blue team), Paint (green), fibers (yellow), drugs (red) Find a true fluorescent detection method published and present it weds 1.Describe the excitation and emmission spectra of the target 2.Describe the light source used to excite the target and the detector used 3.Provide an example of the output 4.Describe any problems or need to optimize detection (interferences?) 5.Describe how it is used in forensic science laboratories. 6.Describe the instruments used in your applications

090810 How Do Materials Emit Light- Lecture by Dr. Stone

090810 Powerpoint on Light and Fluorescence Assignments- 1) Reread Chapter 1 in Valeur- Due 091310 2) Read Chapter 2 in Valeur- Due 091310 3) Study for a quiz covering 090110, 090810, chapters 1 and 2 and fluor applications identified by the class- Quiz on 091310 4) With your teammates, identify 3 questions you have from the lecture on 091310 (Dr. Stone's lecture)

090110 PE and Light powerpoint

Review of Physical Evidence and Light powerpoint. Assignments- Due Weds 09/08/10- Required reading: Other references on fluorescence and semen detection Read Chapter 1 in the book.- Pages 1-19. Read the following 2 articles. Write a 3 page summary, 3 questions and 3 answers. Submit by email to Lee by 09/08/10 1200 noon. Bring hard copy to class weds 090810. * Introduction to fluorescence- http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Introduction-to-Fluorescence-Techniques.html *Tutorial on Fluorescence and Fluorescent Instrumentation downloaded from http://fmrc.pulmcc.washington.edu/DOCUMENTS/FMRC299.pdf on 083010

Fluorescence 101 ppt from 091510

Powerpoint on intro to fluorescence from SLee

Greensheet

Updated syllabus

history of fluorescence powerpoint

Powerpoint by Beniamino Barbieri

Dr. Stone's Presentation on Instrumentation - Lee notes

091510 Notes taken on instrumentation powerpoint

semen fluor detection word

semen fluor detection

083010 Powerpoint- Intro Physical Evidence and assignments

Intro to Physical evidence and assignments 1 and 2. Assignment 1- For each team, find one article from a peer reviewed journal on the application of fluorescence in forensic science Email a copy of the article to all students, Lee and Stone along with a 3 paragraph summary In your summary, provide one picture from the article showing the flour application Due Weds 1 Sept by 1200 noon Be prepared to discuss your article and picture Assignment 2- Sign up for Safety Training on – line http://www.sjsu.edu/hr/students/employees/safety_training/lab/ Complete the bloodborne, hazcom and lab safety training Due weds 8 Sept- print last page out and put your name and date on it.