JS 111 Fluorescent Applications in Molecular Bio and Forensic Sci
Time: Monday, Wednesday 1:30pm-245pm
This course covers scientific concepts, methods, practices, instrumentation, interpretation, statistics and court issues of fluorescence applications in molecular biology and forensic science. Atomic and molecular basis of fluorescence, emission, photophysical processes, energy transfer, QA, validation, accreditation, and admissibility will be covered in lectures, hands-on activities/laboratories, videos and guest lectures.
111 Readings from November
Reading from October and November (sent by email)
110810 Dr. Pourmand Guest lecture
Dr. Nader Pourmand advanced methods in forensic dna
111010 SNP Powerpoint
SNP Powerpoint PDF
FRET review article
Fret review article due 101610
Review of DNA PCR, FRET
100410 powerpoint on intro to dna
102010 Multiplex STR PCR powerpoint
102010 Multiplex PCR presentation
Intro to DNA, PCR and qPCR powerpoint
Intro to DNA PCR and qPCR
Laser lecture 3 by Dr. Stone
Laser Lecture 10/11/10
FRET applications powerpoint grad
102510 FRET applications powerpoint
New assignment 091310
1.Group assignments- Blood (blue team), Paint (green), fibers (yellow), drugs (red)
Find a true fluorescent detection method published and present it weds
1.Describe the excitation and emmission spectra of the target
2.Describe the light source used to excite the target and the detector used
3.Provide an example of the output
4.Describe any problems or need to optimize detection (interferences?)
5.Describe how it is used in forensic science laboratories.
6.Describe the instruments used in your applications
090810 How Do Materials Emit Light- Lecture by Dr. Stone
090810 Powerpoint on Light and Fluorescence
1) Reread Chapter 1 in Valeur- Due 091310
2) Read Chapter 2 in Valeur- Due 091310
3) Study for a quiz covering 090110, 090810, chapters 1 and 2 and fluor applications identified by the class- Quiz on 091310
4) With your teammates, identify 3 questions you have from the lecture on 091310 (Dr. Stone's lecture)
090110 PE and Light powerpoint
Review of Physical Evidence and Light powerpoint.
Due Weds 09/08/10- Required reading: Other references on fluorescence and semen detection
Read Chapter 1 in the book.- Pages 1-19.
Read the following 2 articles. Write a 3 page summary, 3 questions and 3 answers.
Submit by email to Lee by 09/08/10 1200 noon. Bring hard copy to class weds 090810.
* Introduction to fluorescence-
*Tutorial on Fluorescence and Fluorescent Instrumentation downloaded from http://fmrc.pulmcc.washington.edu/DOCUMENTS/FMRC299.pdf on 083010
091510 Student Forensic Fluor applications
articles and presentations from students due 091510
Fluorescence 101 ppt from 091510
Powerpoint on intro to fluorescence from SLee
history of fluorescence powerpoint
Powerpoint by Beniamino Barbieri
Dr. Stone's Presentation on Instrumentation - Lee notes
091510 Notes taken on instrumentation powerpoint
semen fluor detection word
semen fluor detection
083010 Powerpoint- Intro Physical Evidence and assignments
Intro to Physical evidence and assignments 1 and 2.
Assignment 1- For each team, find one article from a peer reviewed journal on the application of fluorescence in forensic science
Email a copy of the article to all students, Lee and Stone along with a 3 paragraph summary
In your summary, provide one picture from the article showing the flour application
Due Weds 1 Sept by 1200 noon
Be prepared to discuss your article and picture
Assignment 2- Sign up for Safety Training on – line
Complete the bloodborne, hazcom and lab safety training
Due weds 8 Sept- print last page out and put your name and date on it.